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mouse anti pparγ  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse anti pparγ
    Mouse Anti Pparγ, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-ppar%CE%B3/PPAR%CE%B3+Antibody/pm41927567-743-65-68
    Average 96 stars, based on 2624 article reviews
    mouse anti pparγ - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Lipid synthesis, triggered by PPARγ T166 dephosphorylation, sustains reparative function of macrophages during tissue repair
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For Western blot the following primary antibodies were used: Mouse anti-PPARγ (1:200) , Santa Cruz, Cat sc-7273 Rabbit anti-PPARγ (1:1000), Cell Signaling Technology, Cat# 2443S Rabbit Phospho-PPAR Gamma (S273) (1:1000), Bioss, Cat# bs-4888R Rabbit Phospho-PPAR Gamma (S112) (1:500), Abcam, Cat# ab195925 Rabbit Phospho-Stat3 (Tyr705) (1:2000), Cell Signaling Technology, Cat# 9145 Rabbit anti-STAT3 (1:1000), Bioworld, Cat# BS1336 Rabbit anti-ACC1 (1:4000), proteintech, Cat# 21923-1-AP Rabbit anti-FASN (1:5000), proteintech, Cat# 10624-2-AP Rabbit anti-Lamin A/C (1:1000), Bioworld, Cat# BS1446 Mouse anti-beta-Actin (1:10000), Zen-Bioscience, Cat# 700068 Mouse GAPDH (1:5000), Zen-Bioscience, Cat# 390035 Mouse Phospho-PPAR Gamma (T166) (1:500), PMID: 36329235 The fluorochrome-labeled antibodies used for FACS analysis are: FITC anti-CD45 (1:100), Biolegend, Cat# 157214 APC anti-F4/80 (1:100), Invitrogen, Cat# 17-4801-82 PE anti-CD11b (1:100), Invitrogen, Cat# 12-0112-81 PerCP-Cy5.5 anti-Ly-6G and Ly6C (1:100), BD Biosciences, Cat# 552093 PE anti-CD3e (1:100), Invitrogen, Cat# 12-0031-83 PE anti-CD206 (1:100), Biolegend, Cat# 141705 APC anti-CD163 (1:100), Invitrogen, Cat# 17-1631-82 PE anti-CD80 (1:100), Invitrogen, Cat# 12-0801-81 PE anti-MHC class II (1:100), Invitrogen, Cat# 12-5321-82 7-AAD (1:100) Biolegend Cat# 420403 Validation Mouse anti-PPARγ, mouse, rat and human, WB, IP, IF, IHC(P) and ELISA, (https://www.scbt.com/p/ppargamma-antibody-e-8? requestFrom=search); In this paper for WB.

    Article Title: HMGB1 Regulates Adipocyte Lipolysis via Caveolin-1 Signaling: Implications for Metabolic and Cardiovascular Diseases
    Article Snippet: Goat anti-HMGB1 and anti-aP2, mouse anti-PPARγ, anti-SREBP-1, anti-phospho-ERK, rabbit anti-HSL, anti-CAV1, and anti-ERK antibodies, as well as protein A/G beads, were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: Chrysanthemum indicum L. ethanol extract reduces high-fat diet-induced obesity in mice
    Article Snippet: The primary antibodies, mouse anti-(PPARγ (sc-7273), CEBPα (sc-166258), β-actin (sc-47778) and rabbit anti-PPARα (sc-9000) were purchased from Santa Cruz Biotechnology, Inc., and rabbit anti-FAS (C20G5) from Cell signaling Technology, Inc. (Danvers, MA, USA).

    Article Title: Luteolin attenuates hepatic steatosis and insulin resistance through the interplay between the liver and adipose tissue in mice with diet-induced obesity.
    Article Snippet: Total protein (80∼100 g) was electrophoresed on 10% 9 SDS polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore), blocked, and probed with rabbit anti-CIDEA (cell death-inducing DFFA-like effector A, 1:1000; Abcam), rabbit anti-ACC (acetyl-CoA carboxylase, 1:1000; Cell Signaling), rabbit anti-SREBP1 (sterol regulatory element-binding protein 1, 1 μg/mL; Abcam), rabbit anti-SREBP2 (sterol regulatory element-binding protein 2, 4 μg/mL; Abcam), mouse anti-PPARγ (peroxisome proliferator-activated receptor γ, 1:1000; Santa Cruz Biotech), rabbit anti-SIRT1 (sirtuin 1, 1:1000; Cell Signaling), and mouse anti-β-actin (1:1000; Cell signaling), respectively.

    Binding Assay:

    Article Title: Lipid synthesis, triggered by PPARγ T166 dephosphorylation, sustains reparative function of macrophages during tissue repair
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For Western blot the following primary antibodies were used: Mouse anti-PPARγ (1:200) , Santa Cruz, Cat sc-7273 Rabbit anti-PPARγ (1:1000), Cell Signaling Technology, Cat# 2443S Rabbit Phospho-PPAR Gamma (S273) (1:1000), Bioss, Cat# bs-4888R Rabbit Phospho-PPAR Gamma (S112) (1:500), Abcam, Cat# ab195925 Rabbit Phospho-Stat3 (Tyr705) (1:2000), Cell Signaling Technology, Cat# 9145 Rabbit anti-STAT3 (1:1000), Bioworld, Cat# BS1336 Rabbit anti-ACC1 (1:4000), proteintech, Cat# 21923-1-AP Rabbit anti-FASN (1:5000), proteintech, Cat# 10624-2-AP Rabbit anti-Lamin A/C (1:1000), Bioworld, Cat# BS1446 Mouse anti-beta-Actin (1:10000), Zen-Bioscience, Cat# 700068 Mouse GAPDH (1:5000), Zen-Bioscience, Cat# 390035 Mouse Phospho-PPAR Gamma (T166) (1:500), PMID: 36329235 The fluorochrome-labeled antibodies used for FACS analysis are: FITC anti-CD45 (1:100), Biolegend, Cat# 157214 APC anti-F4/80 (1:100), Invitrogen, Cat# 17-4801-82 PE anti-CD11b (1:100), Invitrogen, Cat# 12-0112-81 PerCP-Cy5.5 anti-Ly-6G and Ly6C (1:100), BD Biosciences, Cat# 552093 PE anti-CD3e (1:100), Invitrogen, Cat# 12-0031-83 PE anti-CD206 (1:100), Biolegend, Cat# 141705 APC anti-CD163 (1:100), Invitrogen, Cat# 17-1631-82 PE anti-CD80 (1:100), Invitrogen, Cat# 12-0801-81 PE anti-MHC class II (1:100), Invitrogen, Cat# 12-5321-82 7-AAD (1:100) Biolegend Cat# 420403 Validation Mouse anti-PPARγ, mouse, rat and human, WB, IP, IF, IHC(P) and ELISA, (https://www.scbt.com/p/ppargamma-antibody-e-8? requestFrom=search); In this paper for WB.

    Article Title: HMGB1 Regulates Adipocyte Lipolysis via Caveolin-1 Signaling: Implications for Metabolic and Cardiovascular Diseases
    Article Snippet: Goat anti-HMGB1 and anti-aP2, mouse anti-PPARγ, anti-SREBP-1, anti-phospho-ERK, rabbit anti-HSL, anti-CAV1, and anti-ERK antibodies, as well as protein A/G beads, were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: Chrysanthemum indicum L. ethanol extract reduces high-fat diet-induced obesity in mice
    Article Snippet: The primary antibodies, mouse anti-(PPARγ (sc-7273), CEBPα (sc-166258), β-actin (sc-47778) and rabbit anti-PPARα (sc-9000) were purchased from Santa Cruz Biotechnology, Inc., and rabbit anti-FAS (C20G5) from Cell signaling Technology, Inc. (Danvers, MA, USA).

    Article Title: Luteolin attenuates hepatic steatosis and insulin resistance through the interplay between the liver and adipose tissue in mice with diet-induced obesity.
    Article Snippet: Total protein (80∼100 g) was electrophoresed on 10% 9 SDS polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore), blocked, and probed with rabbit anti-CIDEA (cell death-inducing DFFA-like effector A, 1:1000; Abcam), rabbit anti-ACC (acetyl-CoA carboxylase, 1:1000; Cell Signaling), rabbit anti-SREBP1 (sterol regulatory element-binding protein 1, 1 μg/mL; Abcam), rabbit anti-SREBP2 (sterol regulatory element-binding protein 2, 4 μg/mL; Abcam), mouse anti-PPARγ (peroxisome proliferator-activated receptor γ, 1:1000; Santa Cruz Biotech), rabbit anti-SIRT1 (sirtuin 1, 1:1000; Cell Signaling), and mouse anti-β-actin (1:1000; Cell signaling), respectively.



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    Image Search Results


    Effects of chlorogenic acid on adipogenesis. Here, 3T3-L1 adipocytes were treated with H 2 O 2 (100 μM) on days 5, 6, and 7 of the differentiation process for three hours, followed by CGA exposure (5, 10, or 20 μM). Cells cultured with the differentiation medium containing the CGA vehicle alone (0.1% v / v DMSO) were used as controls. ( A ) Representative images of Oil Red O staining (original magnification at ×40—scale bar 50 µm) in control cells (CTR) and in cells exposed to H 2 O 2 and treated or not with CGA. ( B ) Cell lipid accumulation was expressed as relative content vs. control cells. ( C ) PPARγ protein expression was analyzed by Western blot. The densitometry results are reported as fold changes compared to control cells. The values were normalized to the corresponding β-actin value. ( D ) FASN gene expression value was expressed as 2 −ΔΔCt and normalized against control cells. 18S rRNA was used as a housekeeping gene. All results are reported as the mean ± S.D. of three independent experiments (n = 3 biological replicates). Error bars represent S.D. a p < 0.01 vs. control cells; c p < 0.01 vs. H 2 O 2 ; d p < 0.05 vs. H 2 O 2 + CGA 5 μM; e p < 0.01 vs. H 2 O 2 + CGA 5 μM; f p < 0.05 vs. H 2 O 2 + CGA 10 μM.

    Journal: Molecules

    Article Title: Effects of Chlorogenic Acid on Cellular Senescence in an In Vitro Model of 3T3-L1 Murine Adipocytes

    doi: 10.3390/molecules31010167

    Figure Lengend Snippet: Effects of chlorogenic acid on adipogenesis. Here, 3T3-L1 adipocytes were treated with H 2 O 2 (100 μM) on days 5, 6, and 7 of the differentiation process for three hours, followed by CGA exposure (5, 10, or 20 μM). Cells cultured with the differentiation medium containing the CGA vehicle alone (0.1% v / v DMSO) were used as controls. ( A ) Representative images of Oil Red O staining (original magnification at ×40—scale bar 50 µm) in control cells (CTR) and in cells exposed to H 2 O 2 and treated or not with CGA. ( B ) Cell lipid accumulation was expressed as relative content vs. control cells. ( C ) PPARγ protein expression was analyzed by Western blot. The densitometry results are reported as fold changes compared to control cells. The values were normalized to the corresponding β-actin value. ( D ) FASN gene expression value was expressed as 2 −ΔΔCt and normalized against control cells. 18S rRNA was used as a housekeeping gene. All results are reported as the mean ± S.D. of three independent experiments (n = 3 biological replicates). Error bars represent S.D. a p < 0.01 vs. control cells; c p < 0.01 vs. H 2 O 2 ; d p < 0.05 vs. H 2 O 2 + CGA 5 μM; e p < 0.01 vs. H 2 O 2 + CGA 5 μM; f p < 0.05 vs. H 2 O 2 + CGA 10 μM.

    Article Snippet: Membranes were then blocked using 5% non-fat dry milk solution and incubated overnight at 4 °C with the following specific primary antibodies: rabbit anti-Lamin B1 monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA) (1:1000), mouse anti-PPAR-γ monoclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA) (1:1500), rabbit anti-PI3K p85 monoclonal antibody (Cell Signaling Technology) (1:1000), mouse anti-p21 (Waf1/Cip1) monoclonal antibody (Santa Cruz Biotechnology) (1:500), mouse anti-phospho-p53 (Ser15) monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-phospho-p38 (Thr180/Tyr182) monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-phospho-ERK1/2 (Thr202/Tyr204) monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-Bax monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-Bcl-2 monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-Phospho-Akt (Ser473) monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-GLUT-4 monoclonal antibody (Cell Signaling Technology) (1:1000), rabbit anti-NF-κB p65 polyclonal antibody (Invitrogen, Milan, Italy) (1:1000), mouse anti-COX-2 monoclonal antibody (Santa Cruz Biotechnology) (1:500), and rabbit anti-β-actin monoclonal antibody (Cell Signaling Technology) (1:6000).

    Techniques: Cell Culture, Staining, Control, Expressing, Western Blot, Gene Expression